Download - Redaktionssysteme 2011
»Redaktionssysteme«
oyen.de | 2011
KW So. 26/08/12
im Detail Mögliche Wege von Daten, über Layout zu unterschiedlichen KW So. 26/08/12
EckdatenGründung oyen.de2001
Schwerpunkt als Berater, Trainer & Medienprogrammierer Lösungsfindung für Publishing Herausforderungen
Schwerpunkte als Autor Berichterstattung über Trends, Software und Technologien die das systemgestützte Publizieren in die Ausgabekanäle Print, Web und mobile Endgeräte beeinflussen [Multi Channel Publishing]
0/3
Veröffentlichungen (Auszug 2011-2007)
2011☐ Umfrage: Trends 2011 • Publishing & Kommunikation • Medienkanäle & Formate | Oyen Dez 2010 - Jan 2011
2010☐ Multichannel Publishing für Kataloge, Zeitungen und Zeitschriften (PP Nov/Dez 2010) ☐ Multi Format Publishing | iPad Publishing - Von Print zur digitalen Seitengestaltung (PP Nov/Dez 2010) ☐ Multi Format Publishing | Quark XPress für Blio - Print Content multimedial anreichern (PP Sep/Okt 2010) ☐ Multi Format Publishing | Content goes Online: Adobe Indesign als Editor von Webseiten benutzen (PP Jul/Aug 2010) ☐ Definition Multi Format Publishing (Jun 2010)☐ Geschäftsmodelle und Grundregeln beim Aufbereiten von Print-Content für E-Book-Titel (PP Mai/Jun 2010) ☐ Der Weg zum Neugeschäft: Mit »altem« Content auf mobilen Endgeräten (PP Mar/Apr 2010) ☐ [ ] checklist publishing | market overview HTML | PDF (Feb 2010) ☐ Informations-Management: Multi-Channel-Publishing für Print, Web, Devices zweiter Teil - Ausgabe und Trends bei Publishing-Systemen (PP Jan/Feb 2010)
2009☐ Informations-Management: Multi-Channel-Publishing für Print, Web, Devices erster Teil - Input und Verarbeitung mit Publishing-Systemen (PP Nov/Dez 2009)☐ Database Publishing mit Adobe InDesign CS4 und QuarkXPress 8 - ein Testbericht (PP Jul/Aug 2009)☐ Vom Publishing 1.0 zum Publishing 3.0 – die Katalogproduktion geht neue Wege [FR US] (The Dynamic Publisher Mar 2009)☐ E-Book - Technologie, Geräte u. Datenformate (PP Jan/Feb 2009)☐ Multi-Channel-Publishing: Systeme für die Redaktion von morgen (PP Jan/Feb 2009)☐ 2009 Die Zukunft ist da - über den Umgang mit Medienbrüchen (Jan 2009)☐ Medienbruch - ein deutsches (Un)wort und seine Bedeutung im Publishing Umfeld (Jan 2009) 2008☐ Vier Säulen | Database Publishing Integration (Okt 2008)☐ DIN 16511 Korrekturzeichnen für PDF Dokumente (Sep 2008)☐ TCS | Single Source / MultiChannel Publishing (Mai 2008) (technische kommunikation tk 3/08) 2007 ☐ Tools für technische Dokumentation (Nov 2007)☐ Print-to-Web | Vorstellung des Redaktionssystemes K4 | PDF (Aug 2007)☐ Adobe CS3 | Paket für mediale Grenzgänger (Jul 2007) (technische kommunikation tk 4/07)☐ Catalog Trends 2007 Outtake ( Jun 2007)☐ Verteiltes Arbeiten im Netz mit Adobe InDesign (Mai 2007)☐ Das Adobe Creative Suite 3 Universum / Grafik (Mai 2007)☐ Flash und PDF - wenn Dokumente mobil und Inhalte interaktiv werden (Jan 2007)
... kompletter Auszug -> http://oyen.de/press
KW So. 26/08/12
Zeitstrahl
ePaper & mobile Devices
Einführung DBP 1. InDesign Konferenz
webasierte Werbemit-telprodu-ktion via W2P
DBP InBetween / Einrichtung / Training & Markteinführung
2010 2009 2007 2005 2004 2001 1999 1994 1986
Print2iPad / digital
Publishing
Katalog-
trends Print goes Web /
Multi Channel
Publishing / Collabo-
ration
1. DBP
Marktüber-sicht Adobe
InDesign / QuarkX-
Press
CD-ROM Kataloge auf PDF-
Basis / BluePrint
Search&Order
DTP mit
Aldus Pagemaker
0/4
KW So. 26/08/12
PRINT WEB
DEVICES
PIM
PublishingSysteme
Gestalten & Veredeln
AusgabeDatenbereit-stellung
CRM
ResearchMaFo
MRM
Medien Information Manager Vehikel
MAM
360° Publishing Blick
0/5
E-Mailing
Newsletter
Magalog
Geschäftsberichte
Pressemitteilungen
KundenmagazineBücher
Zeitung Magazin
Handbuch
Katalog /Preisliste
Handbuch
BroschürenAnzeige
POSPOS
eBook
mobile
Spielconsolen
smartphone
Kiosk*
Katalog /Preisliste
eBook
mobile
Spiel-konsolen
TV Radio
smartphone
Bücher
Zeitung Magazin Geschäfts-berichte
Presse-mitteilungen
Kunden-magazine
BroschürenAnzeigeVerpackung
Kiosk*
Handbuch
E-Mailing
Webshop
Produktfinder
SocialMedia
Newsletter
POS
Magalog
PRINT WEB
DEVICES
Werbemittel
Marken
Kommunikation
Prod
ukt
Information & Unterhaltung
Verla
g
PR / Unternehmens -
Kom
mun
ikat
ion
E-Mailing
Newsletter
E-Mailing
Newsletter
mobileiPadTablet
KW So. 26/08/12
Redaktionssysteme
1) Der Weg von Daten über Layout zur Ausgabe
2) Systemansätze und Überschneidungen
3) flexibles Layout ./. automatisiertes Layout
4) Komponenten
5) Zusammenarbeit
0/6
Folien 0/1 - 0/6 oyen.de 1/7 - 1/9 Der Weg von Daten über Layout zur Ausgabe2/10 - 2/15 Programmiervorlagen & Publishing Prozesse3/16 - 3/35 Umsetzungen (Auszug) 2002 - 20114/37 - 4/38 Teamaufstellung5/39 - 5/40 Methode & Vorgehen
KW So. 26/08/12
Der Weg von Daten über Layout zur Ausgabe
1
KW So. 26/08/12
Ausgabe (Kanal)
Katalog
Magalog
Magazin
Anzeige
Newsletter
Broschüre
Preisliste
Geschäftsberichte
POS
Verpackung
Dokumentation
� Web
WebShop
Portal
Konfigurator
Newsletter
� mobileEndgeräte
iPad
Tablet
Smartphone
eBook
KIOSK
Daten
� Bereitstellung
� Abgleichen
� Sammeln
� Filtern
� Aufbereiten
� Vervollständigen
Layout
� Programmierung Vorlagen
Beispiele
Zuweisungen
Ergebnis
Seiten / Objekte Layout
Daten / Asset Verknüpfung
Ausgabeformate Print
Web
mobile Endgeräte
RegelwerkeSeitenaufbau / Umbuch
Objektaufbau / Verwendung
Stilvorgaben Absatz
Zeichen
Grep
� Abstimmung /Freigabe
Korrekturworkflow
Statusüberwachung
Remote Publishing / web2Print
� AdaptionFormate
Seiten
Sprachen
Kanal
� ÜbernahmeDaten
Import CSV / XML
http:// Request
ODBC
� ZusammenstellungDokumenten für Ausgabe
� Web
� mobileEndgeräte
� AktualisierungInhalte
bidirektional
� Konvertierung Seitenformate
JPEG
PDF Print
Web
Office
PNG
� Umsetzung
Seitenaufbau (Print)
Aufbereitung mit Daten
Verknüpfen mit Daten und Assets aus Dateisystem
� Export Inhalte
RTF
XML
� Erstellung TOC / Index / Querverweise
Seitenverweise
Sprungziele Web/URL
Neuer Knoten
� Multimediales Anreichern
SlideShow
Audio / Video Player
Interaktion- Animation - Anwendung- E-Mail / URL- Schaltflächen / Navigation- Formulare
Web-Inhalte
Popup-Windows
scrollbare Bereiche
Reflow-Inhalte (ePub /iPad) oyen.de | 2011
Daten > Layout > Ausgabe
1/8
KW So. 26/08/12
Ausgabe (Kanal)
Katalog
Magalog
Magazin
Anzeige
Newsletter
Broschüre
Preisliste
Geschäftsberichte
POS
Verpackung
Dokumentation
� Web
WebShop
Portal
Konfigurator
Newsletter
� mobileEndgeräte
iPad
Tablet
Smartphone
eBook
KIOSK
Daten
� Bereitstellung
� Abgleichen
� Sammeln
� Filtern
� Aufbereiten
� Vervollständigen
Layout
� Programmierung Vorlagen
� Abstimmung /Freigabe
� AdaptionFormate
� ÜbernahmeDaten
� ZusammenstellungDokumenten für Ausgabe
� Web
� mobileEndgeräte
� AktualisierungInhalte
� Konvertierung Seitenformate
� Umsetzung
� Export Inhalte
� Erstellung TOC / Index / Querverweise
� Multimediales Anreichern
Ausgabe (Kanal)
Katalog
Magalog
Magazin
Anzeige
Newsletter
Broschüre
Preisliste
Geschäftsberichte
POS
Verpackung
Dokumentation
� Web
WebShop
Portal
Konfigurator
Newsletter
� mobileEndgeräte
iPad
Tablet
Smartphone
eBook
KIOSK
Daten
� Bereitstellung
� Abgleichen
� Sammeln
� Filtern
� Aufbereiten
� Vervollständigen
Layout
� Programmierung Vorlagen
Beispiele
Zuweisungen
Ergebnis
Seiten / Objekte Layout
Daten / Asset Verknüpfung
Ausgabeformate Print
Web
mobile Endgeräte
RegelwerkeSeitenaufbau / Umbuch
Objektaufbau / Verwendung
Stilvorgaben Absatz
Zeichen
Grep
� Abstimmung /Freigabe
Korrekturworkflow
Statusüberwachung
Remote Publishing / web2Print
� AdaptionFormate
Seiten
Sprachen
Kanal
� ÜbernahmeDaten
Import CSV / XML
http:// Request
ODBC
� ZusammenstellungDokumenten für Ausgabe
� Web
� mobileEndgeräte
� AktualisierungInhalte
bidirektional
� Konvertierung Seitenformate
JPEG
PDF Print
Web
Office
PNG
� Umsetzung
Seitenaufbau (Print)
Aufbereitung mit Daten
Verknüpfen mit Daten und Assets aus Dateisystem
� Export Inhalte
RTF
XML
� Erstellung TOC / Index / Querverweise
Seitenverweise
Sprungziele Web/URL
Neuer Knoten
� Multimediales Anreichern
SlideShow
Audio / Video Player
Interaktion- Animation - Anwendung- E-Mail / URL- Schaltflächen / Navigation- Formulare
Web-Inhalte
Popup-Windows
scrollbare Bereiche
Reflow-Inhalte (ePub /iPad) oyen.de | 2011
Daten > Layout > Ausgabe
1/9
KW So. 26/08/12
Systemansätze und Überschneidungen
2
KW So. 26/08/12
2/11
Systemansätze und Überschneidungen ...Web Content Management Systemkomplexe Verwaltung von Inhalten auf Internetseiten, kann in Richtung Printausgabe erweitert werden
Printproduktionssystemeffektive Erstellung von Printprodukten mittels Erweiterungen in Desktop Publishing Programmen, lösen sich von Print-orientierung
Media Asset Managementsystem(Universelle) Verwaltung von Bildern, Grafiken und Produktionsdaten. Bieten Workflow- und Publishingfunktionalitäten bis hin zur Printausgabe
KW So. 26/08/12
flexibles Layout ./. automatisiertes Layout
3
KW So. 26/08/12
3/13
flexibles Layout ./. automatisiertes LayoutText um Bild schreibenMedienneutrale und redundanzfreie Speicherung der Inhalte erfolgt im Nachgang
Ausgabe auf KnopfdruckFokus liegt auf Verwaltung, Finden von Informationen, sichern von Fakten und Mehrfachverwendung von (Teil)inhalten.
KW So. 26/08/12
InDesign Doppelseite
autom. Mitteilung / Statusaktion aus InDesign
- Dokumentensperre- Teilsperren für Preise etc.- Verzeichnisüberwachung
05 Layout Status
1. Anmerkungen auf Layoutvorschau (Bild JPEG/PDF) von a) Layout b) Text c) Bild
-> unabhängig von InDesign erstellen -> einblenden und abarbeiten erfolgt in InDesign
2. Speichern in WIS3. Freigaben
01 Annotierung
a) Artikeltextvariantenb) Verwendungc) Übergabe Manuskript Urtext / Änderungen Rücklesen
02 Texte
für D-A-CHa) Preiseb) Landesspezifische Layoutelemente
-> Befüllung per Workflow-> Übergabe Korrekte Best-Nr. Preis, Maße, etc.
03 Platzhalter
a) Top / Top-five (Vignetten) / Serienbilderc) Logosd) Stimmungsbilder
-> Bilder für Litho sammeln-> Palette Briefing Bilder
04 Bilder Pull
WIS 2.x System
- Seitenplanung a) Übernahme b) Neugestaltung c) neue Angebote
- Briefings a) Layout b) Text c) Bild d) Angebote e) Zusammen- stellungen
- Abstimmungen- Korrekturen
- Verwendung- Bereitstellung
Push
3/14
KW So. 26/08/12
Chapter 1-6
P ein Produkt pro Seite
01
Cover / Umschlag
Rücken
Cover
01
Rückseite
02
Vorwort
03
Rückseite
04
Vorlaufseiten (Grau I) römisch nummeriert
01
new
products
02
product literature & support material
03
TOC all chapters
04
List of Intern. Represent
01 Chapter
Num &
Section
index (TOC
small)
02
SectionIntro
04
SonderSeiten
Anhang (Grau II) arabisch nummeriert
01
Trade-
marks
02
Sonder-seiten
03
Patent & LicenceDisclaimer
04
Product Index A-Z
06
Product Index 0-9
07
Terms & Conditions
Faxanforderung
6
103
Product 4711ApplicationThe RTS Wheat Germ Linear Template Generation Set, His6-tag can be used to rapidly generate linear expression constructs by PCR, ready for use as DNA templates in RTS 100 Wheat Germ CECF and RTS 500 Wheat Germ CECF protein expression reactions. The use of PCR instead of subcloning into vectors allows rapid protein expression from wild-type or modified DNA sequences. This method of generating DNA templates for protein ex-pression avoids the time-consuming step of cloning into expression plasmids and can be used for initial, rapid screening for expression.
Benefits■ Rapidly create linear expression constructs.■ Optimize regulatory elements.■ Save time; no need for laborious cloning.■ Take advantage of flexible positioning of His6-tag.■ Benefit by adding epitope-tag sequences for detection with antibodies.■ Flexibly introduce desired mutations, change codon usage and generate
truncated proteins.
Product DescriptionIn order to generate linear expression constructs, two PCRs must be per-formed. In the first reaction, gene-specific primers are used to add overlap regions to the sequence of the target gene. The product of the first PCR is mixed with two flanking primers and DNA fragments coding for the T7 pro-motor, eukaryotic 5’- and 3’-regulatory elements and a His6-tag sequence (C-terminal or N-terminal). In the second overlap extension PCR, the prod-uct of the first PCR anneals with the added DNA fragments and the 5’ and 3’ ends are extended. This linear expression construct is then amplified via the flanking primers.
Background InformationOne of the major advantages of cell-free compared to cell-based expression systems is the ability to use PCR-generated templates. Using the RTS Wheat Germ Linear Template Generation Set, His6-tag, if primers are designed for the first (gene specific) of the two PCRsaccording to the guidelines provided in the pack insert, the resulting product can be used in a second PCR to pro-duce a completely functional linear expression template for expression in either the RTS 100 E. coli HY Kit (using RTS E. coli Linear Template Genera-tion Sets) or the RTS 100 Wheat Germ CECF Kit (using the RTS Wheat Germ Linear Template Generation Set). Primers for the second PCR are provided in the RTS Wheat Germ Linear Template Generation Set, His6-tag. The de-sign of these kits enables easy transfer of products from any expression sys-tem to both the RTS Wheat Germ and E. coli platforms, as well as between the two RTS systems. At a later step in the RTS workflow, insertion of these products into an RTS pIVEX Wheat Germ vector may be required (e.g., to produce a template for expression scale-up to RTS 500 Wheat Germ CECF, or to ensure that a well-characterized [sequenced] and stabilized template is used in a series of subsequent experiments).
Ordering and product information, or technical support:www.pharma-enhance-science.comSubscriptions for electronic Pharmaica, product spotlights and more:www.pharma-enhance-science.com/enewsInformation on products available in bulk: scbio.pharma.com
BestellungenGebührenfrei: 0800/235-3152Fax: 0621/235-3136Online Shop: www.IBuyPharma.de
LightCycler 480 SystemInstrument
Rea
l-Ti
me
PC
R S
yste
ms
Rea
l-Ti
me
PC
R
Cat. No. Pack Size Price03 728 790 001 1 set 214,50
(96 reactions)
03 728 790 001 1 set 214,50 (96 reactions)
03 728 790 001 1 set 214,50 (96 reactions)
03 728 790 001 1 set 214,50 (96 reactions)
Figure 1: Principle of the generation of PCR products for in vitro expression using RTS Linear Template Generation Sets. The introduction of an N-terminal His6-tag is shown. For detailed information on the mechanism, refer to the RTS website (www.proteinexpression.com → RTS Wheat Germ Products → Linear Templates and Vectors for both N- and C-terminal tagging).
Contents1. Forward Primer2. Reverse Primer3. C-terminal His6-tag DNA, coding for a C-terminal His6-tag and all
regulatory elements necessary for in vitro protein expression4. N-terminal His6-tag DNA, coding for an N-terminal His6-tag and
all regulatory elements necessary for in vitro protein expression5. Water, PCR-grade
Additional ProductsProduct 5088, see page 123
6
103
Product 4711ApplicationThe RTS Wheat Germ Linear Template Generation Set, His6-tag can be used to rapidly generate linear expression constructs by PCR, ready for use as DNA templates in RTS 100 Wheat Germ CECF and RTS 500 Wheat Germ CECF protein expression reactions. The use of PCR instead of subcloning into vectors allows rapid protein expression from wild-type or modified DNA sequences. This method of generating DNA templates for protein ex-pression avoids the time-consuming step of cloning into expression plasmids and can be used for initial, rapid screening for expression.
Benefits■ Rapidly create linear expression constructs.■ Optimize regulatory elements.■ Save time; no need for laborious cloning.■ Take advantage of flexible positioning of His6-tag.■ Benefit by adding epitope-tag sequences for detection with antibodies.■ Flexibly introduce desired mutations, change codon usage and generate
truncated proteins.
Product DescriptionIn order to generate linear expression constructs, two PCRs must be per-formed. In the first reaction, gene-specific primers are used to add overlap regions to the sequence of the target gene. The product of the first PCR is mixed with two flanking primers and DNA fragments coding for the T7 pro-motor, eukaryotic 5’- and 3’-regulatory elements and a His6-tag sequence (C-terminal or N-terminal). In the second overlap extension PCR, the prod-uct of the first PCR anneals with the added DNA fragments and the 5’ and 3’ ends are extended. This linear expression construct is then amplified via the flanking primers.
Background InformationOne of the major advantages of cell-free compared to cell-based expression systems is the ability to use PCR-generated templates. Using the RTS Wheat Germ Linear Template Generation Set, His6-tag, if primers are designed for the first (gene specific) of the two PCRsaccording to the guidelines provided in the pack insert, the resulting product can be used in a second PCR to pro-duce a completely functional linear expression template for expression in either the RTS 100 E. coli HY Kit (using RTS E. coli Linear Template Genera-tion Sets) or the RTS 100 Wheat Germ CECF Kit (using the RTS Wheat Germ Linear Template Generation Set). Primers for the second PCR are provided in the RTS Wheat Germ Linear Template Generation Set, His6-tag. The de-sign of these kits enables easy transfer of products from any expression sys-tem to both the RTS Wheat Germ and E. coli platforms, as well as between the two RTS systems. At a later step in the RTS workflow, insertion of these products into an RTS pIVEX Wheat Germ vector may be required (e.g., to produce a template for expression scale-up to RTS 500 Wheat Germ CECF, or to ensure that a well-characterized [sequenced] and stabilized template is used in a series of subsequent experiments).
Ordering and product information, or technical support:www.pharma-enhance-science.comSubscriptions for electronic Pharmaica, product spotlights and more:www.pharma-enhance-science.com/enewsInformation on products available in bulk: scbio.pharma.com
BestellungenGebührenfrei: 0800/235-3152Fax: 0621/235-3136Online Shop: www.IBuyPharma.de
LightCycler 480 SystemInstrument
Rea
l-Ti
me
PC
R S
yste
ms
Rea
l-Ti
me
PC
R
Cat. No. Pack Size Price03 728 790 001 1 set 214,50
(96 reactions)
03 728 790 001 1 set 214,50 (96 reactions)
03 728 790 001 1 set 214,50 (96 reactions)
03 728 790 001 1 set 214,50 (96 reactions)
Figure 1: Principle of the generation of PCR products for in vitro expression using RTS Linear Template Generation Sets. The introduction of an N-terminal His6-tag is shown. For detailed information on the mechanism, refer to the RTS website (www.proteinexpression.com → RTS Wheat Germ Products → Linear Templates and Vectors for both N- and C-terminal tagging).
Contents1. Forward Primer2. Reverse Primer3. C-terminal His6-tag DNA, coding for a C-terminal His6-tag and all
regulatory elements necessary for in vitro protein expression4. N-terminal His6-tag DNA, coding for an N-terminal His6-tag and
all regulatory elements necessary for in vitro protein expression5. Water, PCR-grade
Additional ProductsProduct 5088, see page 123
P Produkt über 2 Seiten
02
6
103
Product 4711ApplicationThe RTS Wheat Germ Linear Template Generation Set, His6-tag can be used to rapidly generate linear expression constructs by PCR, ready for use as DNA templates in RTS 100 Wheat Germ CECF and RTS 500 Wheat Germ CECF protein expression reactions. The use of PCR instead of subcloning into vectors allows rapid protein expression from wild-type or modified DNA sequences. This method of generating DNA templates for protein ex-pression avoids the time-consuming step of cloning into expression plasmids and can be used for initial, rapid screening for expression.
Benefits■ Rapidly create linear expression constructs.■ Optimize regulatory elements.■ Save time; no need for laborious cloning.■ Take advantage of flexible positioning of His6-tag.■ Benefit by adding epitope-tag sequences for detection with antibodies.■ Flexibly introduce desired mutations, change codon usage and generate
truncated proteins.
Product DescriptionIn order to generate linear expression constructs, two PCRs must be per-formed. In the first reaction, gene-specific primers are used to add overlap regions to the sequence of the target gene. The product of the first PCR is mixed with two flanking primers and DNA fragments coding for the T7 pro-motor, eukaryotic 5’- and 3’-regulatory elements and a His6-tag sequence (C-terminal or N-terminal). In the second overlap extension PCR, the prod-uct of the first PCR anneals with the added DNA fragments and the 5’ and 3’ ends are extended. This linear expression construct is then amplified via the flanking primers.
Background InformationOne of the major advantages of cell-free compared to cell-based expression systems is the ability to use PCR-generated templates. Using the RTS Wheat Germ Linear Template Generation Set, His6-tag, if primers are designed for the first (gene specific) of the two PCRsaccording to the guidelines provided in the pack insert, the resulting product can be used in a second PCR to pro-duce a completely functional linear expression template for expression in either the RTS 100 E. coli HY Kit (using RTS E. coli Linear Template Genera-tion Sets) or the RTS 100 Wheat Germ CECF Kit (using the RTS Wheat Germ Linear Template Generation Set). Primers for the second PCR are provided in the RTS Wheat Germ Linear Template Generation Set, His6-tag. The de-sign of these kits enables easy transfer of products from any expression sys-tem to both the RTS Wheat Germ and E. coli platforms, as well as between the two RTS systems. At a later step in the RTS workflow, insertion of these products into an RTS pIVEX Wheat Germ vector may be required (e.g., to produce a template for expression scale-up to RTS 500 Wheat Germ CECF, or to ensure that a well-characterized [sequenced] and stabilized template is used in a series of subsequent experiments).
Ordering and product information, or technical support:www.pharma-enhance-science.comSubscriptions for electronic Pharmaica, product spotlights and more:www.pharma-enhance-science.com/enewsInformation on products available in bulk: scbio.pharma.com
BestellungenGebührenfrei: 0800/235-3152Fax: 0621/235-3136Online Shop: www.IBuyPharma.de
LightCycler 480 SystemInstrument
Rea
l-Ti
me
PC
R S
yste
ms
Rea
l-Ti
me
PC
R
Cat. No. Pack Size Price03 728 790 001 1 set 214,50
(96 reactions)
03 728 790 001 1 set 214,50 (96 reactions)
03 728 790 001 1 set 214,50 (96 reactions)
03 728 790 001 1 set 214,50 (96 reactions)
Figure 1: Principle of the generation of PCR products for in vitro expression using RTS Linear Template Generation Sets. The introduction of an N-terminal His6-tag is shown. For detailed information on the mechanism, refer to the RTS website (www.proteinexpression.com → RTS Wheat Germ Products → Linear Templates and Vectors for both N- and C-terminal tagging).
Contents1. Forward Primer2. Reverse Primer3. C-terminal His6-tag DNA, coding for a C-terminal His6-tag and all
regulatory elements necessary for in vitro protein expression4. N-terminal His6-tag DNA, coding for an N-terminal His6-tag and
all regulatory elements necessary for in vitro protein expression5. Water, PCR-grade
Additional ProductsProduct 5088, see page 123
6
103
Product 4711ApplicationThe RTS Wheat Germ Linear Template Generation Set, His6-tag can be used to rapidly generate linear expression constructs by PCR, ready for use as DNA templates in RTS 100 Wheat Germ CECF and RTS 500 Wheat Germ CECF protein expression reactions. The use of PCR instead of subcloning into vectors allows rapid protein expression from wild-type or modified DNA sequences. This method of generating DNA templates for protein ex-pression avoids the time-consuming step of cloning into expression plasmids and can be used for initial, rapid screening for expression.
Benefits■ Rapidly create linear expression constructs.■ Optimize regulatory elements.■ Save time; no need for laborious cloning.■ Take advantage of flexible positioning of His6-tag.■ Benefit by adding epitope-tag sequences for detection with antibodies.■ Flexibly introduce desired mutations, change codon usage and generate
truncated proteins.
Product DescriptionIn order to generate linear expression constructs, two PCRs must be per-formed. In the first reaction, gene-specific primers are used to add overlap regions to the sequence of the target gene. The product of the first PCR is mixed with two flanking primers and DNA fragments coding for the T7 pro-motor, eukaryotic 5’- and 3’-regulatory elements and a His6-tag sequence (C-terminal or N-terminal). In the second overlap extension PCR, the prod-uct of the first PCR anneals with the added DNA fragments and the 5’ and 3’ ends are extended. This linear expression construct is then amplified via the flanking primers.
Background InformationOne of the major advantages of cell-free compared to cell-based expression systems is the ability to use PCR-generated templates. Using the RTS Wheat Germ Linear Template Generation Set, His6-tag, if primers are designed for the first (gene specific) of the two PCRsaccording to the guidelines provided in the pack insert, the resulting product can be used in a second PCR to pro-duce a completely functional linear expression template for expression in either the RTS 100 E. coli HY Kit (using RTS E. coli Linear Template Genera-tion Sets) or the RTS 100 Wheat Germ CECF Kit (using the RTS Wheat Germ Linear Template Generation Set). Primers for the second PCR are provided in the RTS Wheat Germ Linear Template Generation Set, His6-tag. The de-sign of these kits enables easy transfer of products from any expression sys-tem to both the RTS Wheat Germ and E. coli platforms, as well as between the two RTS systems. At a later step in the RTS workflow, insertion of these products into an RTS pIVEX Wheat Germ vector may be required (e.g., to produce a template for expression scale-up to RTS 500 Wheat Germ CECF, or to ensure that a well-characterized [sequenced] and stabilized template is used in a series of subsequent experiments).
Ordering and product information, or technical support:www.pharma-enhance-science.comSubscriptions for electronic Pharmaica, product spotlights and more:www.pharma-enhance-science.com/enewsInformation on products available in bulk: scbio.pharma.com
BestellungenGebührenfrei: 0800/235-3152Fax: 0621/235-3136Online Shop: www.IBuyPharma.de
LightCycler 480 SystemInstrument
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Cat. No. Pack Size Price03 728 790 001 1 set 214,50
(96 reactions)
03 728 790 001 1 set 214,50 (96 reactions)
03 728 790 001 1 set 214,50 (96 reactions)
03 728 790 001 1 set 214,50 (96 reactions)
Figure 1: Principle of the generation of PCR products for in vitro expression using RTS Linear Template Generation Sets. The introduction of an N-terminal His6-tag is shown. For detailed information on the mechanism, refer to the RTS website (www.proteinexpression.com → RTS Wheat Germ Products → Linear Templates and Vectors for both N- and C-terminal tagging).
Contents1. Forward Primer2. Reverse Primer3. C-terminal His6-tag DNA, coding for a C-terminal His6-tag and all
regulatory elements necessary for in vitro protein expression4. N-terminal His6-tag DNA, coding for an N-terminal His6-tag and
all regulatory elements necessary for in vitro protein expression5. Water, PCR-grade
Additional ProductsProduct 5088, see page 123
P mehre Produkte pro Seite
03
4
326
To place an order: 0800 262 1640 orwww.roche-applied-science.com
Technical inquiries: 800 262 4911e-mail: [email protected]
Restriction Enzymes
Mol
ecul
ar B
iolo
gyM
appi
ng a
nd C
loni
ng o
f Nuc
leic
Aci
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Asp EI
Asp EI
Asp EI
Asp EI
from Methanococcus aeolicus PL-15/H
Compatible Ends: None. Isoschizomers: Tth111 I.Methylation Sensitivity: No information available.Activity in PCR buffer: 0%.Pwo SuperYield DNA Polymerase# PCR Mix: 85%; if supplementedwith GC-RICH Solution: 100%. #not available in U.S.Temperature Optimum: +37°CIsoschizomers: Tth111 I.Heat inactivation: No information available.
from Methanococcus aeolicus PL-15/H
Compatible Ends: None. Isoschizomers: Tth111 I.Methylation Sensitivity: No information available.Activity in PCR buffer: 100%Temperature Optimum: +37°CIsoschizomers: Tth111 I.Heat inactivation: No information available.
from Methanococcus aeolicus PL-15/H
Compatible Ends: None. Isoschizomers: Tth111 I.Methylation Sensitivity: No information available.Activity in PCR buffer: 0%.Pwo SuperYield DNA Polymerase# PCR Mix: 85%; if supplementedwith GC-RICH Solution: 100%. #not available in U.S.Temperature Optimum: +37°CIsoschizomers: Tth111 I.Heat inactivation: No information available.
from Methanococcus aeolicus PL-15/H
Compatible Ends: None. Isoschizomers: Tth111 I.Methylation Sensitivity: No information available.Activity in PCR buffer: 100%Temperature Optimum: +37°CIsoschizomers: Tth111 I.Heat inactivation: No information available.
Cat. No. Pack Size Price 11 131 354 001 400 U 64,50
(10 U/µl)
Cat. No. Pack Size Price 11 131 354 001 400 U 64,50
(10 U/µl)
Cat. No. Pack Size Price 11 131 354 001 400 U 64,50
(10 U/µl)
Cat. No. Pack Size Price 11 131 354 001 400 U 64,50
(10 U/µl)
ContentsEnzyme solution supplied with SuRE/Cut Buffer BRestriction Site: GACN↓NNGTC
ContentsEnzyme solution supplied with SuRE/Cut Buffer BRestriction Site: GACN↓NNGTC
ContentsEnzyme solution supplied with SuRE/Cut Buffer BRestriction Site: GACN↓NNGTC
ContentsEnzyme solution supplied with SuRE/Cut Buffer BRestriction Site: GACN↓NNGTC
BufferA B L M H50 to 75% 100% 25 to 50% 75 to 100% 75 to 100%
BufferA B L M H50 to 75% 100% 25 to 50% 75 to 100% 75 to 100%
BufferA B L M H50 to 75% 100% 25 to 50% 75 to 100% 75 to 100%
BufferA B L M H50 to 75% 100% 25 to 50% 75 to 100% 75 to 100%
4
326
To place an order: 0800 262 1640 orwww.roche-applied-science.com
Technical inquiries: 800 262 4911e-mail: [email protected]
Restriction Enzymes
Mol
ecul
ar B
iolo
gyM
appi
ng a
nd C
loni
ng o
f Nuc
leic
Aci
ds
Asp EI
Asp EI
Asp EI
Asp EI
from Methanococcus aeolicus PL-15/H
Compatible Ends: None. Isoschizomers: Tth111 I.Methylation Sensitivity: No information available.Activity in PCR buffer: 0%.Pwo SuperYield DNA Polymerase# PCR Mix: 85%; if supplementedwith GC-RICH Solution: 100%. #not available in U.S.Temperature Optimum: +37°CIsoschizomers: Tth111 I.Heat inactivation: No information available.
from Methanococcus aeolicus PL-15/H
Compatible Ends: None. Isoschizomers: Tth111 I.Methylation Sensitivity: No information available.Activity in PCR buffer: 100%Temperature Optimum: +37°CIsoschizomers: Tth111 I.Heat inactivation: No information available.
from Methanococcus aeolicus PL-15/H
Compatible Ends: None. Isoschizomers: Tth111 I.Methylation Sensitivity: No information available.Activity in PCR buffer: 0%.Pwo SuperYield DNA Polymerase# PCR Mix: 85%; if supplementedwith GC-RICH Solution: 100%. #not available in U.S.Temperature Optimum: +37°CIsoschizomers: Tth111 I.Heat inactivation: No information available.
from Methanococcus aeolicus PL-15/H
Compatible Ends: None. Isoschizomers: Tth111 I.Methylation Sensitivity: No information available.Activity in PCR buffer: 100%Temperature Optimum: +37°CIsoschizomers: Tth111 I.Heat inactivation: No information available.
Cat. No. Pack Size Price 11 131 354 001 400 U 64,50
(10 U/µl)
Cat. No. Pack Size Price 11 131 354 001 400 U 64,50
(10 U/µl)
Cat. No. Pack Size Price 11 131 354 001 400 U 64,50
(10 U/µl)
Cat. No. Pack Size Price 11 131 354 001 400 U 64,50
(10 U/µl)
ContentsEnzyme solution supplied with SuRE/Cut Buffer BRestriction Site: GACN↓NNGTC
ContentsEnzyme solution supplied with SuRE/Cut Buffer BRestriction Site: GACN↓NNGTC
ContentsEnzyme solution supplied with SuRE/Cut Buffer BRestriction Site: GACN↓NNGTC
ContentsEnzyme solution supplied with SuRE/Cut Buffer BRestriction Site: GACN↓NNGTC
BufferA B L M H50 to 75% 100% 25 to 50% 75 to 100% 75 to 100%
BufferA B L M H50 to 75% 100% 25 to 50% 75 to 100% 75 to 100%
BufferA B L M H50 to 75% 100% 25 to 50% 75 to 100% 75 to 100%
BufferA B L M H50 to 75% 100% 25 to 50% 75 to 100% 75 to 100%
P Kreuztabelle Doppelseite
04
Publishing System
Publishing System generate InDesign pages
Smile
Indesign Book
03
Productpages(4 Vari.)
Heiler Print
Manager
CMS
PublicationsManager
3/15
KW So. 26/08/12
3/15
KW So. 26/08/12
Komponenten
4
KW So. 26/08/12
4/18
Komponenten .Editorial SystemErstellung und Bearbeitung von Content
-Verwaltungswerkzeuge für Zugriffsunterstützung, Aufgabenverteilung und Statusüberwachung
-Verarbeitungswerkzeuge für Contenterzeugung, properitär und/oder integration externen (Text)Editoren
-> Layout-orientiert -> Zeilenfallverbindlich
-> Content-orientiert -> strukturelle inhaltliche Aufbereitung
KW So. 26/08/12
4/19
Komponenten ..Content RepositoryZentrale Speicherung der erstellten Inhalte
-Trennung von Inhalt und Form -> Multi Format Publishing
-Abbildung Makrostrukturen -> Inhaltebausteine referenzieren
-Abbildung Mikrostrukturen -> Textkörper zerlegen
-gleichzeitiger Zugriff verschiedener Benutzer auf Inhalte
-> Check-Out -> Ein Benutzer bearbeitet Inhalt, Inhalt ist für andere Benutzer gesperrt
-> Check-In -> neue Version wird geladen und zur Bearbeitung durch andere Benutzer freigeben
KW So. 26/08/12
4/20
Komponenten ...Publishing SystemAusgabeseite des Content Management Systems
-Printprodukte -> Seitenvorbereitung -> Ausschiessen -> Farbseparierung
-Onlineprodukte-> Granularität der Gestaltung -> Template / StyleSheet / Skript
-> Zeitpunkt der Erzeugung -> statisch oder dynamisch
KW So. 26/08/12
Zusammenarbeit
5
KW So. 26/08/12
1. Planung & Steuerung
Publishing Prozess 3M mit jCatalog PIM & Cross Media PublisherPreislisten / Katalog
2. Kreation
3. Repro
4. Bilddatenmanagement Datenhandling
Vorlagen-erstellung
5. Reinzeichnung
PDF Druckdaten
Layout-abstimmung
Publikations-planung
BildbearbeitungBilderstellung
Bilder / Texte CSV Daten InDesign.-PDF Dokumente
Seitenaufbau Text / Bild Integration
Aktualisierung Abstimmung
FinishingInh.verz. / TOC
Dokumenten-sammlung
Lektorat
PIM
Cross Media Publisher
Daten
Bilder Texte
oyen.de | 2011
CMS
PublicationsManager
5/22
KW So. 26/08/12
5/23
ZusammenarbeitTermin- und AblaufplanungBasis für strukturierte Prozesse und Abläufe -> Eskalationsstufen, Benachrichtigung und Aufgabendelegierung
EchtzeitkommunikationInstant Messaging und Chat
WhiteboardingSkizzieren von Anordnungen, Seitenaufbauten
AnnotierungenErgänzend zu Annotierungen im PDF, webasierte, kollaborative Anmerkungen
KW So. 26/08/12
Danke.
KW So. 26/08/12