Transcript
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pGEMR-T Easy
• Dient zur leichten Klonierungvon PCR-Produkten
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pBluescript, ein klassisches Phagemid
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Substitutionsvektoren
Linker Arm „Stuffer“ Rechter Arm
IntegratLigation
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Sal Bam Eco Eco Bam Sal
Verdau mit Sal I + Eco
Sal Bam Eco Eco Bam Sal
Sal
Sal
Eco
Eco
Sal
Sal
Fällen mit Isopropanol
Eco
Eco
Sal
Sal
Integrat
Linker Arm Rechter Arm„Stuffer“
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Lambda Zap:Moderne Vektoren Mit Phagen und Plasmid-eigenschaften