![Page 1: YNYUR A Y (YYYYY) · (YYYYY) n Branch Point Polypyrimidine Tract ~40 nts YNYUR A Y Intron Exon A B Figure 1 . Intron de˜nition Exon de˜nition Splice-site recognition Exon A Exon](https://reader036.vdokument.com/reader036/viewer/2022071006/5fc3fd74696e0356e17b2f19/html5/thumbnails/1.jpg)
5' splice site
3' splice site
(YYYYY) n
Branch Point Polypyrimidine Tract
~40 nts
YNYUR A Y
Intron
Exon
A
B
Figure 1
![Page 2: YNYUR A Y (YYYYY) · (YYYYY) n Branch Point Polypyrimidine Tract ~40 nts YNYUR A Y Intron Exon A B Figure 1 . Intron de˜nition Exon de˜nition Splice-site recognition Exon A Exon](https://reader036.vdokument.com/reader036/viewer/2022071006/5fc3fd74696e0356e17b2f19/html5/thumbnails/2.jpg)
Intron de�nition Exon de�nition
Splice-site recognition
Exon A Exon B Exon A Exon B
Figure 2
A
Splice-site recognition
Splice-site Pairing
U2
(Long Intron)(Short Intron)
B
U1 U2 A n o x E
B
n o
x E
U1 2 U
2 U
A n o x E
B
n o
x E
U1
1 U
2 U
U2 U1 U1
Spliceosomal Assembly
Splice-site Pairing
![Page 3: YNYUR A Y (YYYYY) · (YYYYY) n Branch Point Polypyrimidine Tract ~40 nts YNYUR A Y Intron Exon A B Figure 1 . Intron de˜nition Exon de˜nition Splice-site recognition Exon A Exon](https://reader036.vdokument.com/reader036/viewer/2022071006/5fc3fd74696e0356e17b2f19/html5/thumbnails/3.jpg)
Figure 3
hnRNPsFox andOthers
ESE
SRProtein
U1U2
ISE
U1U2
ESSISShnRNPPTB and
Others
A
B
![Page 4: YNYUR A Y (YYYYY) · (YYYYY) n Branch Point Polypyrimidine Tract ~40 nts YNYUR A Y Intron Exon A B Figure 1 . Intron de˜nition Exon de˜nition Splice-site recognition Exon A Exon](https://reader036.vdokument.com/reader036/viewer/2022071006/5fc3fd74696e0356e17b2f19/html5/thumbnails/4.jpg)
5' splice site
Intron Exon
S S I
Figure 4
Activating Secondary Structure Inhibitory Secondary Structure
ESE
![Page 5: YNYUR A Y (YYYYY) · (YYYYY) n Branch Point Polypyrimidine Tract ~40 nts YNYUR A Y Intron Exon A B Figure 1 . Intron de˜nition Exon de˜nition Splice-site recognition Exon A Exon](https://reader036.vdokument.com/reader036/viewer/2022071006/5fc3fd74696e0356e17b2f19/html5/thumbnails/5.jpg)
Figure 5
variable intron length
variable exon length
Exon/intron architecture
Local Secondary Structure
3' splice site
A (YYYYY)n
5' splice site
ESEESSISE ISS
Branch PointPolypyrimidine Tract
~40 nts
Promoter Poly-adenylation site
![Page 6: YNYUR A Y (YYYYY) · (YYYYY) n Branch Point Polypyrimidine Tract ~40 nts YNYUR A Y Intron Exon A B Figure 1 . Intron de˜nition Exon de˜nition Splice-site recognition Exon A Exon](https://reader036.vdokument.com/reader036/viewer/2022071006/5fc3fd74696e0356e17b2f19/html5/thumbnails/6.jpg)
Figure 6
ISS ISS ISS ISE ESE C U ESS
Inhibitory Secondary Structures
Position 6 C to U SMN2 Switch
3’ Splice Site 5’ Splice Site
variable intron length
Silencing Elements
Enhancing Elements
Exon 7