metzger kirchleitner et al figure file mdsc revision kopie
TRANSCRIPT
Figure S1Spleen
Tumor
Ly6G
Ly6C
PMN-MDSC
M-MDSCCD11b
FSC
-A
Tumor
FSC-A FVD CD45
SS
C-A
FSC-A
FSC
-H
FSC
-A
FSC
-A
FSC-A
SS
C-A
Ly6G
Ly6C
PMN-MDSC
M-MDSCFSC-A
FSC
-H
FVD
FSC
-A
CD45
FSC
-A
CD11b
FSC
-A
NK1.1
CD
3
NK cells
CD3
CD
19
B cells
CD
4
CD8a
F4/8
0
Ly6C
T cells Macrophages
NK1.1
CD
3
CD3
CD
19
CD
4
CD8a
F4/8
0
Ly6C
NK cells B cells T cells TAM
0
5
10
15
20
TumorSpleen
MH
C-I
(MFI
x 1
03 )
GMacrophages/TAM
Figure S2
CD
69+ (
%)
B
** **
A
012345
Ø
NK cells
% o
f CD
45+
poly(I:C)c
02468
10
Ø
NK cells
poly(I:C)c
Spleen Tumor
0
20
40
60
80
Ø
B cells
poly(I:C)c
0
10
20
30
40
Ø
B cells
poly(I:C)c
Spleen Tumor
**
020406080
100
TumorSpleen
B cells
***
020406080
100 NK cells
TumorSpleen
** **
Macrophages
0
2
4
6
8
Ø poly(I:C)c
TAM
0
10
20
30
40
Ø poly(I:C)c
Spleen Tumor
** *
E
% o
f CD
45+
poly(I:C)cUntreated
poly(I:C)cUntreated
01020304050
Ø poly(I:C)c
**
F
D Tumor
0
0.2
0.4
0.6
0.8cDC1
%of
CD
45+
*
0
0.1
0.2
0.3cDC2
0
1
2
3 **
0
1
2
3
Ø poly(I:C)c
Ø poly(I:C)c
Ø poly(I:C)c Ø poly(I:C)c
0
1
2
3
4
00.51.01.52.02.5 **
%of
CD
45+
Ø poly(I:C)c Ø poly(I:C)c
Lymph nodecDC1
cDC2
0
0.1
0.2
0.3
0.4
0
0.05
0.10
0.15
0
0.5
1.0
1.5
0
1
2
3 *
%of
CD
45+
Ø poly(I:C)c
Ø poly(I:C)c
CD
40 (M
FI x
103 )
Ø poly(I:C)c Ø poly(I:C)c
CD
86 (M
FI x
103 )
0
0.5
1.0
1.5
2.0 **
0
2.0
4.0
6.0
8.0 *
%of
CD
45+
Ø poly(I:C)c Ø poly(I:C)c
C
0
2
4
6
8
CD
204+ C
D20
6+ (%
)
Ø poly(I:C)c
% o
f CD
45+
H
020406080
100
-TAM
T ce
ll%
Prol
ifera
tion
Spleen Tumor
Macrophages/TAM
CD
40 (M
FI x
103 )
CD
86 (M
FI x
103 )
CD
40 (M
FI x
103 )
CD
86 (M
FI x
103 )
CD
40 (M
FI x
103 )
CD
86 (M
FI x
103 )
CD
204+ C
D20
6+ (%
)
poly(I:C)c - +- +-
CD4+ T cells CD8+ T cells
Figure S3
A
0
20
40
60
80
100
Pur
ity(%
)
0.0
2.5
5.0
7.5
10.0
RIN
0.001
0.01
0.1
1
10
Krt1
8 / β-a
ctin
0.001
0.01
0.1
1
10
Krt8
/ β-
actin
M-
MD
SC
PM
N-
MD
SC
M-
MD
SC
PM
N-
MD
SC
M-
MD
SC
PM
N-
MD
SC
M-
MD
SC
PM
N-
MD
SC
Spleen Tumor Spleen Tumor
M-
MD
SC
PM
N-
MD
SC
Tumor
Tum
or
M-
MD
SC
PM
N-
MD
SC
Tumor
Tum
or
B
D
cell cycleRNA splicing
mitotic nuclear divisioncell division
mRNA processingmRNA splicing, via spliceosome
inflammatory responsecellular response to interferon-beta
cilium morphogenesispositive regulation of transcription from RNA polymerase II promoter
mitotic cytokinesiscilium assembly
positive regulation of gene expressiondefense response
negative regulation of transcription, DNA-templatedmicrotubule-based movement
cell adhesionnegative regulation of transcription from RNA polymerase II promoter
innate immune responsepositive regulation of cell proliferation
-log10(adj. p-value)
adj. p-value: 0.000032
0 5 10 15
PMN-MDSC
M-MDSC
C
Spleen
Spleen
320 80100
471 113 97
Tumor
Tumor
Figure S4
Stfa2l1Fpr1Hsd11b1Pglyrp1Wfdc21H2−T24Trim34aOas1bGlrxMov10Trim30aRtp4Oas3Cmpk2Oas2Slc28a2Ifi27l2aUsp18Slfn5Slfn8Ifit3Ifit3bGm20559Rsad2Ddx58Irf7Ddx60Gm43197Sp100Ly6c2Slfn4Isg20Helz2DaxxOasl1Plac8Zbp1Stat2Isg15Ifitm3Ifi35Tap1Trafd1Ube2l6Oas1gXaf1Ifit1bl1Znfx1Dhx58Rnf213Oas1aC3Phf11aIfi44Mx1Ifi209Phf11bIfi213Ifi211Ifi207PmlCcl5Bst2Ly6aLgals3bpPhf11dIrgm1Ifi47Gm12250Fcgr4Ifi214Ly6iTrim30cH2−T10H2−T22Ly6eB2mMx2Parp12Ifi206AC238811.2Herc6Ifi204Ifi208Ifit2H2−Q7H2−Q5H2−Q4H2−Q6H2−K1MatkCiitaKmoAhnakSlc1a5Ccr2H2−AaH2−Eb1H2−Ab1Cd74
SpleenTumor
PMN−MDSC (n=100)
−2 −1 0 1 2
Row Z−score
AhnakArhgap26Prkag2Kbtbd11Pbx1Padi2AhrTrerf1Uck2Plcb1Itga1Fn1Kdm7aSt8sia4Dhcr24Ern1Raph1Plcl1Trps1CfhAlcamMatkTiparpGab1Fosl2Ifit1Isg20KdrIfit1bl1Usp18Slfn4Ifi208Ifit2Rsad2Oasl1Ifi44CfbPhf11dBst2Il2rgOas1gP2ry13Oasl2Ddx58Rtp4Znfx1Tor3aMov10Phf11bH2−T22Ifit3Irf7Peli1Ifit3bFpr2Serpina3gTgtp1Cyth3Stra6lNr1h3Pla2g7Ddx60Gm6545Slfn9Slc28a2Ifi206Ifi213Gm15931Endod1Cmpk2Ly6iBC147527Parp11Irgm2GnsGrnAoahLair1AW112010Card11Dnase1l3GpnmbFmnl2Cd38Fcgr4Acp5CtsbAdap2Zfand2aRgs1Fth1Acp2Pik3ap1Iigp1Gm4951AdgbSiglec1Cd274Ccnd2Acod1Ly6aH2−Q4H2−Q7H2−Q6Gbp6P2ry14Fpr1H2−K1Lgals3bpIl18bpEbi3Stk39B2m
M−MDSC (n=113)
−2 −1 0 1 2
Row Z−score
poly(I:C)c- +- + poly(I:C)c
- +- +