reporter gene imaging. using antibody fragments as artificial receptors ligands antibody fragments...
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Reporter Gene Imaging
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Using antibody fragments as artificial “receptors”
Ligands
Antibody fragments
Membraneanchoringdomains
Hapten
-X*
Hapten
1-X
*
Hapten
2-Y
*
AB
Vector
mRNA
DNA
mRNA mRNA
tissuespecificpromoter
Constitutivepromoter
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Antibody reporter DAbR1
U87-DAbR1
0
10
20
30
40
50
60
HSVtk/FHBG
Reporter Gene / Reporter Probe
%ID
/g
tumor+ tumor-
blood
DAbR1/AABD
Liver
PET/CT with Y86-DOTAComparison with HSVtk reporter
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Translational Molecular Imaging
Hohe Sensitifität (pmol/l)Nachweis von RezeptorenMöglich
Minimalste Stoffmengen!Keine pharmakologischenEffekte
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Untersuchung der myokardialen Perfusion und des Sauerstoffverbrauchs mit 11C-1-acetate
Ng et al. Am J Physiol 1994
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Sun et al. J Nucl Med 1998
Untersuchung der myokardialen Perfusion und des Sauerstoffverbrauchs mit 11C-1-acetate
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Bengel et al.EJNM 2000
Untersuchung der myokardialen Effizienz mit 11C-1-acetate
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Multimodale PET/CT Bildgebung
LAD Stenose ohne Ischämie
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Multimodale PET/CT Bildgebung
LCX Stenose mit ausgeprägter Ischämie
? Antibody deficiency: CVID, XLA, HIM, subclass deficiency, sIgA" href="https://vdokument.com/normale-infektanfaelligkeit-und-immundefizienz-beim-differentialdiagnose-der.html">Normale Infektanfälligkeit und Immundefizienz beim … · Differentialdiagnose* der ID im Erwachsenenalter 6."3).7,/>? Antibody deficiency: CVID, XLA, HIM, subclass deficiency, sIgA