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bbe Moldaenke GmbH, Preetzer Chaussee 177, 24222 Schwentinental, Germany Page 1

Do you know the high sensitive ToxProtect?

Fish in a tank - best choice for a

simple, affordable and effective Early

Warning System

12th Webinar

bbe Moldaenke GmbH, Preetzer Chaussee 177, 24222 Schwentinental, Germany Page 2

Welcome

bbe Team

Detlev Lohse Ylva Tischler

bbe Moldaenke GmbH, Preetzer Chaussee 177, 24222 Schwentinental, Germany Page 3

12th Webinar

Part 1 Why do we measure Fish toxicity?

Part 2 What is the Value of ToxProtect?

Part 3 Construction & Function

Part 4 Application

Conclusion

Feedback

Follow up

bbe Moldaenke GmbH, Preetzer Chaussee 177, 24222 Schwentinental, Germany Page 4

The Testorganism in the Phylogenetic Tree

Part 1

bbe Moldaenke GmbH, Preetzer Chaussee 177, 24222 Schwentinental, Germany Page 5

OPPTS 850.1075 EPA

Test: ACUTE TOXICITY FOR FISH

The purpose of this test is to

determine the acute lethal toxicity of a

substance to fish in fresh water and

marine water.

bbe Moldaenke GmbH, Preetzer Chaussee 177, 24222 Schwentinental, Germany Page 6

Literature on Fish Toxicity

…..may cause fish and

aquatic invertebrate

deaths [43].

Chlorpyrifos toxicity to

fish may be related to

water temperature. The

96-hour LC50 for

chlorpyrifos is 0.009

mg/L in mature rainbow

trout, 0.098 mg/L in lake

trout, 0.806 mg/L in

goldfish, 0.01 mg/L in

bluegill, and 0.331 mg/L

in fathead minnow

[50]……

bbe Moldaenke GmbH, Preetzer Chaussee 177, 24222 Schwentinental, Germany Page 7

How does the Static Test work?

bbe Moldaenke GmbH, Preetzer Chaussee 177, 24222 Schwentinental, Germany Page 8

Is the Static Test an Option?

Why not?

bbe Moldaenke GmbH, Preetzer Chaussee 177, 24222 Schwentinental, Germany Page 9

Online Toxicity and Pollution Wave

Toximeter signal

Pollutant concentration

bbe Moldaenke GmbH, Preetzer Chaussee 177, 24222 Schwentinental, Germany Page 10

The Alternative – Chemical Analysis?

bbe Moldaenke GmbH, Preetzer Chaussee 177, 24222 Schwentinental, Germany Page 11

ToxProtect is a cost-effective,

automated biomonitor for rapid

detection of acute toxic substances

in water

detection of fish movements by an array of light

barriers

remote access to data including all optional

sensors and online operation

low maintenance

simple installation

ToxProtect 64

Part 2

bbe Moldaenke GmbH, Preetzer Chaussee 177, 24222 Schwentinental, Germany Page 12

What makes a ToxProtect Attractive in

Toxicity Monitoring?

Continuous monitoring

Ease of operation

Sensitive vertebrate – related physiology

Reliability of hardware

Wide temperature range: cold & warm water fish

bbe Moldaenke GmbH, Preetzer Chaussee 177, 24222 Schwentinental, Germany Page 13

Set-up of the ToxProtect

Part 3

bbe Moldaenke GmbH, Preetzer Chaussee 177, 24222 Schwentinental, Germany Page 14

Essential Parts of the ToxProtect

bbe Moldaenke GmbH, Preetzer Chaussee 177, 24222 Schwentinental, Germany Page 15

Essential Parts of the ToxProtect

bbe Moldaenke GmbH, Preetzer Chaussee 177, 24222 Schwentinental, Germany Page 16

To put the Items together

Hardware ToxProtect

Fish/Testorganism

AlarmSoftware

bbe Moldaenke GmbH, Preetzer Chaussee 177, 24222 Schwentinental, Germany Page 17

Medaka Zebrafish Dace (Leuciscus)

Tigerbarb Blue gill Fathead minnow

To each Place the Suitable Fish

bbe Moldaenke GmbH, Preetzer Chaussee 177, 24222 Schwentinental, Germany Page 18

www.pesticideinfo.org/List_EcoChemSpecies.jsp?Taxa_Group='Fish'

Selection for the Type of Substances

bbe Moldaenke GmbH, Preetzer Chaussee 177, 24222 Schwentinental, Germany Page 19

Response Time

bbe Moldaenke GmbH, Preetzer Chaussee 177, 24222 Schwentinental, Germany Page 20

To put the Items together

Hardware ToxProtect

Fish/Testorganism

Alarm Software

bbe Moldaenke GmbH, Preetzer Chaussee 177, 24222 Schwentinental, Germany Page 21

Activity

Specific activity

Thresholds

Alarm

Alarm Recognition

bbe Moldaenke GmbH, Preetzer Chaussee 177, 24222 Schwentinental, Germany Page 22

Problem:

• false alarms occur due to the natural variation in behaviour

• due to the low risk of an event, alarms can be wrong

Consequence:

• an alarm evaluation is required to reduce the risk

Solutions

• second independent instrument

• second type of test in the same instrument

False Alarm Prevention

bbe Moldaenke GmbH, Preetzer Chaussee 177, 24222 Schwentinental, Germany Page 23

Alarm Verification = False Alarm Prevention

bbe Moldaenke GmbH, Preetzer Chaussee 177, 24222 Schwentinental, Germany Page 24

To put the Items together

Hardware ToxProtect

Fish/Testorganism

Alarm Software

bbe Moldaenke GmbH, Preetzer Chaussee 177, 24222 Schwentinental, Germany Page 25

Real alarm from pig

farm faeces in

Latvia

Application Measuring Station Part 4

bbe Moldaenke GmbH, Preetzer Chaussee 177, 24222 Schwentinental, Germany Page 26

Hydropower dam

upside Tehran, Iran

Application Water Quality Monitoring

bbe Moldaenke GmbH, Preetzer Chaussee 177, 24222 Schwentinental, Germany Page 27

ToxProtect is a qualified tool for water quality

survey. It detects contaminations in short

times.

ToxProtect combines the ability of fish toxity

assessment with a superior alarm

recognition.

ToxProtect includes an alarm verification

check.

ToxProtect reduces costs and risks.

Summary

bbe Moldaenke GmbH, Preetzer Chaussee 177, 24222 Schwentinental, Germany Page 28

Thank you

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